Transcriptome-driven synthetic re-modeling of Escherichia coli to enhance cellobiose utilization

Chemical Engineering Science, Volume 103,  2013, Pages 50-57.

Vinuselvi Parisutham, Sang-Kyu Jung, Dougu Nam, Sung Kuk Lee.

 

School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798, Republic of Korea and

School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798, Republic of Korea.

 

Abstract

An efficient cellobiose-metabolizing strain of Escherichia coli would provide several industrial benefits, particularly with lignocellulosic biomass substrates. An efficient cellobiose-metabolizing E. coli strain (without the introduction of heterologous genes) was constructed by a combination of promoter engineering of cryptic operons (chb and asc) and adaptive evolution. Gene expression patterns in rapid and slow cellobiose-metabolizing strains were analyzed using DNA microarray. Genes involved in nucleotide biosynthesis, ATP generation, and flagellar assembly were upregulated following adaptation, indicating the possibility of active metabolism and fast growth. On the other hand, genes involved in chemotaxis toward glucose and response to starvation and other stresses were downregulated. No significant changes were observed in the expression of genes regulated by cyclic AMP receptor protein (CRP), the global regulator of carbon metabolism. Overexpression of ascB, which is located in the minor cryptic operon, seems to play a vital role in enhancing cellobiose metabolism. Changes in ascB transcription were confirmed by semiquantitative RT-PCR. The importance of ascB for cell growth on cellobiose was also validated by overexpression and knockout studies. Our study provides new insights into the role of the chb and ascoperons in cellobiose metabolism.

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