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Lianzhe Hu, Shuang Han, Zhongyuan Liu, Saima Parveen, Yali Yuan, Guobao Xu
Electrochemistry Communications, Volume 13, Issue 12, December 2011
Abstract
We reported a versatile strategy for electrochemical determination of H2O2 as well as related enzymes and substrates by combining the advantages of H2O2-mediated selective boronate deprotection and p-aminophenol for the first time. H2O2 is selectively detected through the determination of p-aminophenol generated from H2O2-mediated boronate deprotection of p-aminophenylboronic acid. This method allows H2O2 detection in the range from 1 μM to 1 mM with a detection limit of 0.8 μM. Since many enzymes and their substrates can be detected through the determination of H2O2, we extend this method to detect related enzymes and their substrates using glucose and glucose oxidase as representative analytes. There is a good linear relationship between the current responses and GOx activity from 0.01 U/mL to 0.8 U/mL with a detection limit of 0.01 U/mL. The method is promising for broad and important applications, such as driver alcohol detection as well as clinical analysis of glucose, uric acid, lactate, glutamate, and so on.
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