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Sensors and Actuators B: Chemical, Volume 161, Issue 1, 3 January 2012, Pages 1046-1052
Paola Rivolo, Francesco Michelotti, Francesca Frascella, Gabriella Digregorio, Pietro Mandracci, Lorenzo Dominici, Fabrizio Giorgis, Emiliano Descrovi
Dipartimento di Scienza dei Materiali e Ingegneria Chimica, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy
Dipartimento di Scienze di Base ed Applicate per l’Ingegneria, SAPIENZA Università di Roma, Via A. Scarpa 16, 00161 Roma, Italy
Dipartimento di Fisica, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy
Abstract
We report on time resolved sensing experiments demonstrating the direct, label-free and specific binding of an Antibody/Anti-antibody system probed by means of Bloch surface waves on silicon-based one-dimensional photonic crystals. The selectivity of the device is verified by monitoring the adsorption of Anti Mouse IgG (non-specific target) and Anti Rabbit IgG (specific target) on rabbit IgG receptors previously transferred by micro contact printing on the surface of 1DPC devices. The limits of the sensitivity are explored by varying the concentration of the tested solutions. Based on a modified ELISA assay, we performed a quantitative estimation of the number of detected antibodies adsorbed at the probed surface in both the non-specific and specific case (∼104 and ∼108, respectively), as associated to measured spectral shifts in the sensorgram.
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