Label-free polypeptide-based enzyme detection using a graphene-nanoparticle hybrid sensor.

Adv Mater. 2012 Nov 27;24(45):6081-7.

Myung S, Yin PT, Kim C, Park J, Solanki A, Reyes PI, Lu Y, Kim KS, Lee KB.

Department of Chemistry and Chemical Biology, Institute for Advanced Materials Devices, and Nanotechnology (IAMDN), Rutgers The State University of New Jersey, Piscataway, NJ 08854, USA.

 

Abstract

 

A graphene-nanoparticle (NP) hybrid biosensor that utilizes an electrical hysteresis change to detect the enzymatic activity and concentration of Carboxypeptidase B was developed. The results indicate that the novel graphene-NP hybrid biosensor, utilizing electrical hysteresis, has the ability to detect concentrations of targeted enzyme on the micromolar scale. Furthermore, to the knowledge of the authors, this is the first demonstration of a graphene-based biosensor that utilizes a hysteresis change resulting from metallic NPs assembled on a graphene surface.

Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

 

 

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